Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways . Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop . LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin , and stabilizing the actin cytoskeleton . In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation . Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly . Stimulates axonal outgrowth and may be involved in brain development . ; [Isoform 3]: Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin .

LIM domain kinase 1 (LIMK-1) (EC 2.7.11.1)

p53667

Human

WB

WB 1:1000-1:5000

~73 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.