Non-enzymatic adapter protein that plays a pivotal role in precisely regulated signaling cascades from cell surface receptors to cellular responses, including signaling transduction and gene expression . Thus, participates in many biological processes including regulation of innate and adaptive immunity, autophagy, DNA repair or necroptosis . Controls signaling complexes at the T-cell antigen receptor to facilitate the activation, differentiation, and function of T-cells . Mechanistically, engagement of the TCR leads to phosphorylation of the adapter protein LAT, which serves as docking site for GRB2 . In turn, GRB2 establishes a a connection with SOS1 that acts as a guanine nucleotide exchange factor and serves as a critical regulator of KRAS/RAF1 leading to MAPKs translocation to the nucleus and activation . Functions also a role in B-cell activation by amplifying Ca mobilization and activation of the ERK MAP kinase pathway upon recruitment to the phosphorylated B-cell antigen receptor . Plays a role in switching between autophagy and programmed necrosis upstream of EGFR by interacting with components of necrosomes including RIPK1 and with autophagy regulators SQSTM1 and BECN1 . Regulates miRNA biogenesis by forming a functional ternary complex with AGO2 and DICER1 . Functions in the replication stress response by protecting DNA at stalled replication forks from MRE11-mediated degradation. Mechanistically, inhibits RAD51 ATPase activity to stabilize RAD51 on stalled replication forks . Additionally, directly recruits and later releases MRE11 at DNA damage sites during the homology-directed repair process . ; [Isoform 2]: Does not bind to phosphorylated epidermal growth factor receptor but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. Mechanistically, inhibits RAS-ERK signaling and downstream cell proliferation by competing with GRB2 for SOS1 binding and thus by regulating SOS1 membrane recruitment .

Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)

p62993

Human, Mouse, Rat

WB,IHC,IF,ICC,IP

WB 1:2000
IHC 1:100
IF 1:100
ICC 1:100
IP 1:20

~26 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.