[Isoform 1]: Transcription factor that is selectively and transiently expressed in cleavage-stage embryos . Binds to double-stranded DNA elements with the consensus sequence 5'-TAATCTAATCA-3' . Binds to chromatin containing histone H3 acetylated at 'Lys-27' and promotes deacetylation of H3K27ac. In parallel, binds to chromatin that lacks histone H3 acetylation at 'Lys-27' and recruits EP300 and CREBBP to promote acetylation of histone H3 at 'Lys-27' at new sites . Involved in transcriptional regulation of numerous genes, primarily as transcriptional activator, but also mediates repression of a set of target genes . Promotes expression of ZSCAN4 and KDM4E, two proteins with essential roles during early embryogenesis . Promotes nuclear translocation of CTNNB1/beta-catenin and its subsequent activation of target genes . Heterologous expression in cultured embryonic stem cells mediates transcription of HERVL retrotransposons and transcripts derived from ACRO1 and HSATII satellite repeats . May activate expression of PITX1 . May regulate microRNA expression . Inappropriate expression can inhibit myogenesis and promote apoptosis . ; [Isoform 2]: Probably inactive as a transcriptional activator, due to the absence of the C-terminal region that is important for transcriptional activation. Can inhibit transcriptional activation mediated by isoform 1. Heterologous expression of isoform 2 has no deleterious effect on cell survival.

Double homeobox protein 4 (Double homeobox protein 10)

q9ubx2

Human

WB

WB 1:1000-1:5000

~45 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.