Functions in mitochondrial and peroxisomal division . Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism . The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes . While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane . Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner . Plays an important role in mitochondrial fission during mitosis . Required for formation of endocytic vesicles . Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage . Required for normal brain development, including that of cerebellum . Facilitates developmentally regulated apoptosis during neural tube formation . Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues . Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles . Required for programmed necrosis execution . Rhythmic control of its activity following phosphorylation at Ser-637 is essential for the circadian control of mitochondrial ATP production . ; [Isoform 14]: Regulates postsynaptic clathrin-mediated endocytosis by positioning the endocytic zone at the postsynaptic density, independently of mitochondrial division.

Dynamin-1-like protein (EC 3.6.5.5) (Dynamin family member proline-rich carboxyl-terminal domain less) (Dymple) (Dynamin-related protein 1)

o00429,q8k1m6

Human,Mouse,Rat

WB,IHC,IF,ICC,IP,FC

WB 1:1000IHC 1:200-1:1000IF 1:100-1:200ICC 1:100-1:200IP 1:20-1:50FC 1:50-1:100

~82 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.