Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences . Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts and tRNA-like molecules . Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome . G-quadruplexes are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA .

G-rich sequence factor 1 (GRSF-1)

q12849

Human,Mouse,Rat

WB,IF,ICC,IP,FC

WB 1:1000
IF 1:100-1:200
ICC 1:100-1:200
IP 1:50
FC 1:100

~54 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.