Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle . Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers . Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis . Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion . Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly . The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization . Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis . Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center . May be an activator of PLCE1 . In neurons, involved in the inhibition of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling . Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets . When activated by DAAM1 may signal centrosome maturation and chromosomal segregation during cell division. May also be involved in contractile ring formation during cytokinesis. ; Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague.

Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)

p61586

Human,Mouse,Rat

WB,IF,ICC,FC

WB 1:1000-1:5000
IF 1:100
ICC 1:100
FC 1:100-1:200

~22 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.