TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton . Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ . The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation . Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells . Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling . With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus . May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions .

Tight junction protein 1 (Tight junction protein ZO-1) (Zona occludens protein 1) (Zonula occludens protein 1)

q07157

Human,Mouse,Rat

IHC,IF,ICC,FC

IHC 1:200-1:500
IF 1:100
ICC 1:100
FC 1:50-1:100

~196 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.