Multifunctional polyribosome-associated RNA-binding protein that plays a central role in neuronal development and synaptic plasticity through the regulation of alternative mRNA splicing, mRNA stability, mRNA dendritic transport and postsynaptic local protein synthesis of target mRNAs . Acts as an mRNA regulator by mediating formation of some phase-separated membraneless compartment: undergoes liquid-liquid phase separation upon binding to target mRNAs, leading to assemble mRNAs into cytoplasmic ribonucleoprotein granules that concentrate mRNAs with associated regulatory factors . Plays a role in the alternative splicing of its own mRNA . Stabilizes the scaffolding postsynaptic density protein DLG4/PSD-95 and the myelin basic protein mRNAs in hippocampal neurons and glial cells, respectively; this stabilization is further increased in response to metabotropic glutamate receptor stimulation . Plays a role in selective delivery of a subset of dendritic mRNAs to synaptic sites in response to mGluR activation in a kinesin-dependent manner . Undergoes liquid-liquid phase separation following phosphorylation and interaction with CAPRIN1, promoting formation of cytoplasmic ribonucleoprotein granules that concentrate mRNAs with factors that inhibit translation and mediate deadenylation of target mRNAs . Acts as a repressor of mRNA translation in synaptic regions by mediating formation of neuronal ribonucleoprotein granules and promoting recruitmtent of EIF4EBP2 . Plays a role as a repressor of mRNA translation during the transport of dendritic mRNAs to postsynaptic dendritic spines . Component of the CYFIP1-EIF4E-FMR1 complex which blocks cap-dependent mRNA translation initiation . Represses mRNA translation by stalling ribosomal translocation during elongation . Reports are contradictory with regards to its ability to mediate translation inhibition of MBP mRNA in oligodendrocytes . Also involved in the recruitment of the RNA helicase MOV10 to a subset of mRNAs and hence regulates microRNA -mediated translational repression by AGO2 . Facilitates the assembly of miRNAs on specific target mRNAs . Also plays a role as an activator of mRNA translation of a subset of dendritic mRNAs at synapses . In response to mGluR stimulation, FMR1-target mRNAs are rapidly derepressed, allowing for local translation at synapses . Binds to a large subset of dendritic mRNAs that encode a myriad of proteins involved in pre- and postsynaptic functions . Binds to 5'-ACU[GU]-3' and/or 5'-[AU]GGA-3' RNA consensus sequences within mRNA targets, mainly at coding sequence and 3'-untranslated region and less frequently at 5'-UTR . Binds to intramolecular G-quadruplex structures in the 5'- or 3'-UTRs of mRNA targets . Binds to G-quadruplex structures in the 3'-UTR of its own mRNA . Also binds to RNA ligands harboring a kissing complex structure; this binding may mediate the association of FMR1 with polyribosomes . Binds mRNAs containing U-rich target sequences . Binds to a triple stem-loop RNA structure, called Sod1 stem loop interacting with FMRP , in the 5'-UTR region of superoxide dismutase SOD1 mRNA . Binds to the dendritic, small non-coding brain cytoplasmic RNA 1 ; which may increase the association of the CYFIP1-EIF4E-FMR1 complex to FMR1 target mRNAs at synapses . Plays a role in mRNA nuclear export . Specifically recognizes and binds a subset of N6-methyladenosine -containing mRNAs, promoting their nuclear export in a XPO1/CRM1-dependent manner . Together with export factor NXF2, is involved in the regulation of the NXF1 mRNA stability in neurons . Associates with export factor NXF1 mRNA-containing ribonucleoprotein particles in a NXF2-dependent manner . Binds to a subset of miRNAs in the brain . May associate with nascent transcripts in a nuclear protein NXF1-dependent manner . In vitro, binds to RNA homomer; preferentially on poly and to a lesser extent on poly, but not on poly or poly . Moreover, plays a role in the modulation of the sodium-activated potassium channel KCNT1 gating activity . Negatively regulates the voltage-dependent calcium channel current density in soma and presynaptic terminals of dorsal root ganglion neurons, and hence regulates synaptic vesicle exocytosis . Modulates the voltage-dependent calcium channel CACNA1B expression at the plasma membrane by targeting the channels for proteasomal degradation . Plays a role in regulation of MAP1B-dependent microtubule dynamics during neuronal development . Has been shown to play a translation-independent role in the modulation of presynaptic action potential duration and neurotransmitter release via large-conductance calcium-activated potassium channels in hippocampal and cortical excitatory neurons . May be involved in the control of DNA damage response mechanisms through the regulation of ATR-dependent signaling pathways such as histone H2AX/H2A.x and BRCA1 phosphorylations . Forms a cytoplasmic messenger ribonucleoprotein network by packaging long mRNAs, serving as a scaffold that recruits proteins and signaling molecules. This network facilitates signaling reactions by maintaining proximity between kinases and substrates . ; [Isoform 10]: Binds to RNA homomer; preferentially on poly and to a lesser extent on poly, but not on poly or poly . May bind to RNA in Cajal bodies . ; [Isoform 6]: Binds to RNA homomer; preferentially on poly and to a lesser extent on poly, but not on poly or poly . May bind to RNA in Cajal bodies . ; Acts as a positive regulator of influenza A virus replication. Required for the assembly and nuclear export of the viral ribonucleoprotein components.

Fragile X messenger ribonucleoprotein 1 (Fragile X messenger ribonucleoprotein) (FMRP) (Protein FMR-1)

q06787, p35922

Human, Mouse

WB

WB 1:1000

~72 kD

Rabbit

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Store at -20°C. Stable for 12 months from date of receipt.