PW22854

货号 规格 价格(¥)
PW22854S 50μl
PW22854M 100μl

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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   蛋白质印迹

   免疫荧光

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   植物BPP法变性蛋白提取

   组织制备成单细胞悬液

经典一抗-MAPKAPK-2 (Phospho-T334) antibody

Datasheet

Background

In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK. Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay. Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2. Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2.

Alternative Names

MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2; MAPKAPK2

Uniprot

p49137

Reactivity

Human

Application

WB,IF,ICC

Application Image

Recommended Dilution

WB 1:2000-1:5000
IF 1:50-1:200
ICC 1:100-1:400

Mol Weight

~ 48 kDa

Source

Rabbit

Specificity

This antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at Thr334.

Immunogen

Synthetic phosphopeptide derived from human MAPKAPK-2 around the phosphorylation site of Threonine 334.

Clonality

Polyclonal

Isotype

Rabbit IgG

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Concentration

1mg/ml

Storage

Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.

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      微信客服

邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13916964679(微信同号)

技术支持:15618194176(微信同号)

华南经销商负责(广东,广西,福建,海南):
程经理:手机18616261485(微信同号)
华北经销商负责(北京,天津,河北):
徐经理:手机15618191473(微信同号)
南方经销商负责:

陆经理:手机13122837132(微信同号)
北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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      微信客服

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