VPS34 (vacuolar protein sorting-associated protein 34), also known as VPT29, VPL7, PEP15 or END12, is a 875 amino acid peripheral membrane protein that localizes to the golgi apparatus. Belonging to the PI3/PI4-kinase family, VPS34 is involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. The VPS34 PI3-kinase complex II is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes. It has been suggested that VPS34 may also be involved in ethanol tolerance and cell wall integrity.

Phosphatidylinositol 3-kinase catalytic subunit type 3 (EC:2.7.1.137); PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34; PIK3C3;

q8neb9

Human,Mouse,Rat

WB,IP

WB 1:500-1:1000
IP 1:50-1:200

~ 100 kDa

Rabbit

This antibody detects endogenous levels of VPS34 protein only when phosphorylated at Ser249.

Synthetic peptide, corresponding to Human VPS34.

Polyclonal

Rabbit IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1mg/ml

Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.