PW13445

货号 规格 价格(¥)
PW13445S 50μl
PW13445M 100μl

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

ABMART实验方案下载

   蛋白质免疫共沉淀

   蛋白质印迹

   免疫荧光

   免疫组化

   膜再生

   植物BPP法变性蛋白提取

   组织制备成单细胞悬液

经典一抗-eEF2K antibody

Datasheet

Background

eEF2K is 105 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38γ, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.

Alternative Names

Elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; EEF2K

Uniprot

o00418

Reactivity

Human,Mouse,Rat

Application

WB,IHC

Application Image

Recommended Dilution

WB 1:500-1:1000
IHC 1:50-1:200

Mol Weight

~ 105 kDa

Source

Rabbit

Specificity

This antibody detects endogenous levels of eEF2K protein.

Immunogen

Synthetic peptide, corresponding to amino acids 331-380 of Human eEF2K.

Clonality

Polyclonal

Isotype

Rabbit IgG

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Concentration

1mg/ml

Storage

Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.

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北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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      微信客服

邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13916964679(微信同号)

技术支持:15618194176(微信同号)

华南经销商负责(广东,广西,福建,海南):
程经理:手机18616261485(微信同号)
华北经销商负责(北京,天津,河北):
徐经理:手机15618191473(微信同号)
南方经销商负责:

陆经理:手机13122837132(微信同号)
北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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      微信客服

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