The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Two of these family members Six3 and Six6 (also designated Optx2 and Six9) are required for the specification and proliferation of the eye field in vertebrates, and, therefore, are the vertebrate homologues most closely related to the Drosophila sine oculis protein, which has an essential role in controlling compound eye development. Six3 and Six6 expression largely overlap during development of specific tissues, such as retina, hypothalamus, and pituitary. Deletion of Six3 may be associated with HPE2 disorder, a common, severe malformation of the brain that results from incomplete cleavage of the forebrain during early embryogenesis.

Homeobox protein SIX3; Sine oculis homeobox homolog 3; SIX3; HPE2; Homeobox protein SIX6; Homeodomain protein OPTX2; Optic homeobox 2; SIX6; OPTX2; SIX9

o95343/o95475

Human,Mouse,Rat

WB,IF,ICC

WB 1:500-1:1000
IF 1:50-1:200
ICC 1:100-1:400

~ 28 kDa (Six6); 35 kDa (Six3)

Rabbit

This antibody detects endogenous levels of Six3 protein (35 KDa), and also detects Six6 protein (28 KDa).

Synthetic peptide, corresponding to amino acids 201-250 of Human Six3.

Polyclonal

Rabbit IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1mg/ml

Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.