Recoverin is implicated in the regulation of rhodopsin kinase activity that contributes to the adaptation to background illumination in retinal photoreceptor cells. Recoverin, a Ca2+-binding photoreceptor protein, is recognized as an autoantigen of cancer-associated retinopathy (CAR), which is a rare paraneoplastic neurological syndrome characterized by the degeneration of retinal photoreceptors and associated with small-cell lung cancer. Recoverin is a heterogeneously myristoylated protein that inhibits rhodopsin kinase by inhibiting its phosphorylation. Ca2+ is required for Recoverin to bind rhodopsin kinase. In addition, the binding of Recoverin-rhodopsin kinase is weakened by autophosphorylation of the kinase and is strengthened by the presence of ADP. Upon accommodating two Ca2+ ions, Recoverin extrudes a myristoyl group and associates with the lipid bilayer membrane.

Recoverin; Cancer-associated retinopathy protein; Protein CAR; RCVRN; RCV1

p35243

Human,Mouse,Rat

WB,IHC,IF,ICC

WB 1:500-1:1000
IHC 1:50-1:200
IF 1:50-1:200
ICC 1:100-1:400

~ 26 kDa

Rabbit

This antibody detects endogenous levels of Recoverin protein.

Synthetic peptide, corresponding to amino acids 120-160 of Human Recoverin.

Polyclonal

Rabbit IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1mg/ml

Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.