The spliceosome, the 0gigantic molecular machine that performs pre-mRNA splicing in eukaryotes, contains over 200 different proteins and five RNA molecules (U1, U2, U4, U5 and U6). Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The PRP19-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is released. Changes within the spliceosome upon binding of the PRP19-associated complex include remodeling of the U6/5‘ splice site interaction and destabilization of Lsm proteins to allow further interaction of U6 with the intron sequence.

Pre-mRNA-processing factor 19; Nuclear matrix protein 200; PRP19/PSO4 homolog; hPso4; RING-type E3 ubiquitin transferase PRP19; Senescence evasion factor; PRPF19I; NMP200; PRP19; SNEV

q9ums4

Human,Mouse,Rat

WB

WB 1:500-1:1000

~ 55 kDa

Rabbit

This antibody detects endogenous levels of PRPF19 protein.

Synthetic peptide, corresponding Human PRPF19.

Polyclonal

Rabbit IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1mg/ml

Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.