The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with Golgi membranes and the association of actin to the Golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes. Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins, and activation of phospholipase D. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization. The human ARF1 gene maps to chromosome 1q42, contains 5 exons and 4 introns, and encodes a 181 amino acid protein.

ADP ribosylation factor 2; ADP ribosylation factor 4; ARF2;

p18085

Human,Mouse

WB,IHC

WB 1:500-1:1000
IHC 1:50-1:200

~ 21 kDa

Rabbit

This antibody detects endogenous levels of ARF4 protein.

Synthetic peptide, corresponding to amino acids 80-130 of Human ARF4.

Polyclonal

Rabbit IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1mg/ml

Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.