PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction.PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen.

Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4; PAK4; KIAA1142; Serine/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6; PAK-6; PAK6; PAK5; Serine/threonine-protein kinase PAK 7; p21-activated kinase 5; p21-activated kinase 7; PAK-7; PAK7; KIAA1264

o96013/q9nqu5/q9p286

Human,Mouse,Rat

WB,IP

WB 1:500-1:1000
IP 1:10-1:100

~ 64 kDa (PAK4), 80 kDa (PAK5), 75 kDa (PAK6)

Rabbit

This antibody detects endogenous levels of PAK4, PAK5 and PAK6 protein.

Synthetic peptide, corresponding to amino acids 441-490 of Human PAK4.

Polyclonal

Rabbit IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1mg/ml

Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.