Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5'' flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. SREBP-1 (also designated ADD1, for adipocyte determination and differentiation factor) is synthesized as a precursor that is attached to the nuclear envelope and endoplasmic reticulum. In sterol-depleted cells, the membrane-bound precursor is cleaved to generate a soluble NH2-terminal fragment that translocates to the nucleus to activate transcription. Sterols inhibit the cleavage of SREBP-1.

Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1; SREBF1; BHLHD1; SREBP1

p36956

Human,Rat,Mouse

WB

WB 1:500-1:1000

~ 121 kDa

Rabbit

SREBP-1 (phospho-S439) polyclonal antibody detects endogenous levels of SREBP-1 protein only when phosphorylated at Ser439.

Synthetic phosphopeptide derived from human SREBP-1 around the phosphorylation site of Serine 439

Polyclonal

Rabbit IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1mg/ml

Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.