The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway. The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses. CISH/CIS1, the first described member of the SOCS family, is induced by a number of cytokines including IL-2, IL-3, GM-CSF, and EPO. The CISH protein appears as a doublet around 32 and 37 kDa, the nature of which is unknown. CISH binds to phosphorylated cytokine receptors and can inhibit Stat5 activity. Expression of CISH is regulated by Stat5, thereby providing feedback modulation. Transgenic mice overexpressing CISH display phenotypes similar to Stat5 knockouts, including defects in mammary gland development and in T and NK cell regulation. Research studies have shown that polymorphisms within the CISH gene are associated with susceptibility to infectious diseases.

Cytokine-inducible SH2-containing protein; CIS; CIS-1; Protein G18; Suppressor of cytokine signaling; SOCS; CISH; G18

q9nse2

Rat,Mouse

WB,IHC

WB 1:500-1:1000
IHC 1:50-1:200

~ 32 kDa

Rabbit

This antibody detects endogenous levels of CISH protein.

Synthetic peptide, corresponding to Human CISH.

Polyclonal

Rabbit IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1mg/ml

Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.