Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton. Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1. VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability. Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV).
Occludin; OCLN
q16625
Human,Rat,Mouse
WB
WB 1:1000-1:2000
~ 65 kDa
Rabbit
This antibody detects endogenous levels of Occludin protein.
Recombinant fusion protein of human Occludin.
Polyclonal
Rabbit IgG
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
PBS with 0.02% sodium azide, 50% glycerol, pH7.2
1mg/ml
Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.
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