Located in the mitochondrial inner membrane, mitochondrial complex I is the first and largest enzyme in the electron transport chain of oxidative phosphorylation. By oxidizing NADH that is produced in the Krebs cycle, this complex utilizes the two electrons to reduce ubiquinone to ubiquinol, thereby initiating the passage of electrons to successive complexes and ultimately leading to the reduction of oxygen to water. Mitochondrial complex I consists of over 40 subunits and is of considerable clinical interest since defects in any of the subunits can lead to various myopathies and neuropathies. As a subunit of mitochondrial complex I, NDUFS5 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5), also designated NADH-ubiquinone oxidoreductase 15 kDa subunit, is a 106 amino acid protein that is suggested to not be involved in catalysis. NDUFS5 is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart.

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5; Complex I-15 kDa; CI-15 kDa; NADH-ubiquinone oxidoreductase 15 kDa subunit; NDUFS5

o43920

Human

WB,IHC

WB 1:500-1:1000
IHC 1:50-1:200

~ 15 kDa

Rabbit

This antibody detects endogenous levels of NDUFS5 protein.

Synthetic peptide, corresponding to amino acids 61-110 of Human NDUFS5.

Polyclonal

Rabbit IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

PBS with 0.02% sodium azide, 50% glycerol, pH7.2

1mg/ml

Store at 4℃ short term,and store at -20℃ long term. Avoid freeze-thaw cycles.