IGF2 Rabbit mAb_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC85121

货号规格价格(￥)

PC85121S 50ul

PC85121M 100ul

以上产品均有现货供应

订购咨询： orders@ab-mart.com

订货热线： 4006-123-828

技术支持：15618194176

经销商名录：点击查看

Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

ABMART实验方案下载

经典一抗-IGF2 Rabbit mAb

Datasheet

Description

The IGF2 gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is expressed only from the paternally inherited allele and is an imprinted gene which is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3'' region and to the upstream INS gene at the 5'' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types.

Alternative Names

IGF2_HUMAN; Insulin-like growth factor II; IGF-II; IGF II; Somatomedin-A; T3M-11-derived growth factor; Insulin-like growth factor II; Insulin-like growth factor II Ala-25 Del; Preptin; PP1446; Insulin like Growth Factor 2;

Uniprot

p01344

GeneID

3481

Gene Name

IGF2

Application Image

Reactivity

Human

Application

WB,IHC,IF,ICC

Recommended Dilution

WB 1:1000
IHC 1:100-500
IF 1:100-1:400
ICC 1:100-1:400

Mol Weight

11kD

Tissue specificity

Source

Rabbit

Immunogen

Recombinant human IGF2 protein（aa 25-121 /180）

CAS

Clonality

Monoclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Concentrate

1mg/ml

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Western blot analysis of IGF2 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (PC85121, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 20 kDa Observed band size: 11 kDa

	Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-IGF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (PC85121, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-IGF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (PC85121, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IGF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (PC85121, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF2) Monoclonal Antibody, Unconjugated (PC85121) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) A10026 instructionsand DAB staining.