Histone H2A.X Rabbit mAb

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC93425

货号规格价格(￥)

PC93425S 50ul

PC93425M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Histone H2A.X Rabbit mAb

Datasheet

Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008].

Alternative Names

H2AX_HUMAN; 2A.X variant histone; H2A.X; H2AX; H2A/X; H2AFX; Histone H2AX;

Uniprot

p16104

GeneID

3014

Gene Name

H2AX

Application Image

Reactivity

Human,Mouse,Rat

Application

WB,IHC,IF,ICC

Recommended Dilution

WB 1:500-2000
IHC 1:50-200
IF 1:100-1:400
ICC 1:100-1:400

Mol Weight

16kD

Tissue specificity

Source

Rabbit

Immunogen

KLH conjugated synthetic peptide derived from human H2AX

CAS

Clonality

Monoclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Concentrate

1mg/ml

Buffer

0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

25 ug total protein per lane of various lysates (see on figure) probed with Histone H2A.X monoclonal antibody, unconjugated (PC93425) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

	Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H2A.X Monoclonal Antibody, Unconjugated(PC93425) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, A10026) and DAB staining.