Nrf2 Rabbit mAb_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC93438

货号规格价格(￥)

PC93438S 50ul

PC93438M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Nrf2 Rabbit mAb

Datasheet

Description

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription
factor which regulates the expression of many detoxification and
antioxidant enzymes. Nrf2 can potentially play a significant role in adaptive responses to oxidative stress. Nrf2 belongs to the Cap N Collar (CNC-bZIP) subfamily of basic /leucine zipper (bZIP) transcription factors.

Alternative Names

Nuclear factor-E2 related factor2; Nrf-2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN.

Uniprot

q16236

GeneID

4780

Gene Name

NFE2L2

Application Image

Reactivity

Human,Mouse,Rat

Application

WB,IHC,IF,ICC,FC

Recommended Dilution

WB 1:500-2000
IHC 1:100-500
IF 1:100-1:400
ICC 1:100-1:400
FC 1:100

Mol Weight

68kD

Tissue specificity

Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

Source

Rabbit

Immunogen

KLH conjugated synthetic peptide derived from human Nrf2

CAS

Clonality

Monoclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Concentrate

1mg/ml

Buffer

0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

	Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, A10026) and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nrf2 Monoclonal Antibody, Unconjugated(PC93438) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.

Hela (H) cells were treated with or without sodium orthovanadate (10 μM) for 10 h, 25 μg total protein per lane of cell lysates (see on figure) probed with Nrf2 monoclonal antibody, unconjugated (PC93438) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.