eIF4E Rabbit mAb_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC95604

货号规格价格(￥)

PC95604S 50ul

PC95604M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-eIF4E Rabbit mAb

Datasheet

Description

Acts in the cytoplasm to initiate and regulate protein synthesis and is required in the nucleus for export of a subset of mRNAs from the nucleus to the cytoplasm which promotes processes such as RNA capping, processing and splicing.

Alternative Names

CBP; eIF-4E; eIF-4F 25 kDa subunit; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E.

Uniprot

p06730

GeneID

1977

Gene Name

EIF4E

Application Image

Reactivity

Human,Mouse,Rat

Application

WB,IHC,IF,ICC,IP,FC

Recommended Dilution

WB 1:500-2000
IHC 1:50-200
IF 1:100-1:400
ICC 1:100-1:400
IP 1:20-50
FC 1:20-100

Mol Weight

Calculated: 25kD, Observed: 30kD

Tissue specificity

Source

Rabbit

Immunogen

KLH conjugated synthetic peptide derived from human eIF4E

CAS

Clonality

Monoclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Concentrate

Buffer

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.

	Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated(PC95604) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.

	Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated (PC95604) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
	Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated (PC95604) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
	Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated (PC95604) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
	Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with eIF4E Monoclonal Antibody, Unconjugated (PC95604) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

25 ug total protein per lane of various lysates (see on figure) probed with eIF4E monoclonal antibody, unconjugated (PC95604) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.