MCM2 Rabbit mAb_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC95656

货号规格价格(￥)

PC95656S 50ul

PC95656M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-MCM2 Rabbit mAb

Datasheet

Description

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for ''once per cell cycle'' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.

Alternative Names

BM28; CCNL1; cdc19; CDCL1; Cyclin like 1; MCM2.

Uniprot

p49736

GeneID

4171

Gene Name

MCM2

Application Image

Reactivity

Human,Mouse,Rat

Application

WB,IHC,IF,ICC

Recommended Dilution

WB 1:500-2000
IHC 1:100-500
IF 1:50-200
ICC 1:50-200

Mol Weight

Calculated: 102kD, Observed: 125kD

Tissue specificity

Source

Rabbit

Immunogen

KLH conjugated synthetic peptide derived from human MCM2

CAS

Clonality

Monoclonal

Isotype

IgG

Purification

affinity purified by Protein A

Formulation

Liquid

Concentrate

Buffer

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.

	Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Tonsil Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MCM2 Monoclonal Antibody, Unconjugated(PC95656) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.

25 ug total protein per lane of various lysates (see on figure) probed with MCM2 monoclonal antibody, unconjugated (PC95656) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.