Integrin beta 1 Rabbit mAb

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PC95666

货号规格价格(￥)

PC95666S 50ul

PC95666M 100ul

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Integrin beta 1 Rabbit mAb

Datasheet

Description

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi''s sarcoma lesions.

Alternative Names

Integrin beta-1; beta 1 subunit; CD 29; CD-29; CD29; CD29 antigen; Fibrinogen Receptor beta subunit; Fibronectin receptor subunit beta; Fibronectin receptor, beta subunit; FNRB; GPIIA; GP IIa; Integrin VLA 4 subunit beta; ITGB; ITGB1; ITGB 1; MDF2; MSK12; Very Late Activation Protein beta Polypeptide; Glycoprotein IIa; Integrin VLA 4 subunit beta; integrin VLA-4 beta subunit; ITB1_HUMAN; very late activation protein, beta polypeptide; VLA BETA; VLA-4 subunit beta; VLA-BETA; VLAB; VLAbeta; Integrin β1.

Uniprot

p05556

GeneID

3688

Gene Name

ITGB1

Application Image

Reactivity

Human,Mouse,Rat

Application

WB,IHC,IF,ICC

Recommended Dilution

WB 1:500-2000
IHC 1:100-500
IF 1:100-1:400
ICC 1:100-1:400

Mol Weight

88kD

Tissue specificity

Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in peripheral T-cells. Isoform beta-1C is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).

Source

Rabbit

Immunogen

KLH conjugated synthetic peptide derived from human Integrin beta 1

CAS

Clonality

Monoclonal

Isotype

IgG

Purification

Protein A

Formulation

Liquid

Concentrate

1mg/ml

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

	Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin beta 1 Monoclonal Antibody, Unconjugated(PC95666) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin beta 1 Monoclonal Antibody, Unconjugated(PC95666) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin beta 1 Monoclonal Antibody, Unconjugated(PC95666) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin beta 1 Monoclonal Antibody, Unconjugated(PC95666) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin beta 1 Monoclonal Antibody, Unconjugated(PC95666) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin beta 1 Monoclonal Antibody, Unconjugated(PC95666) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin beta 1 Monoclonal Antibody, Unconjugated(PC95666) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin beta 1 Monoclonal Antibody, Unconjugated(PC95666) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin beta 1 Monoclonal Antibody, Unconjugated(PC95666) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin beta 1 Monoclonal Antibody, Unconjugated(PC95666) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
	Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin beta 1 Monoclonal Antibody, Unconjugated(PC95666) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.

25 ug total protein per lane of various lysates (see on figure) probed with Integrin beta 1 monoclonal antibody, unconjugated (PC95666) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.