IRF3 Antibody_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

MG623921

货号规格价格(￥)

MG623921S 50μl

MG623921M 100μl

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-IRF3 Antibody

Datasheet

Description

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:22394562, PubMed:24049179, PubMed:25636800, PubMed:27302953, PubMed:31340999, PubMed:36603579, PubMed:8524823). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:11846977, PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:32972995, PubMed:36603579, PubMed:8524823). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431, PubMed:36603579). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:22394562, PubMed:25636800, PubMed:27302953, PubMed:36603579). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16154084, PubMed:27302953, PubMed:33440148, PubMed:36603579). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed:25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).

Alternative Names

Interferon regulatory factor 3 (IRF-3)
IRF3 interferon regulatory factor 3; MGC94729

UniProt

Q14653

Reactivity

Human, Monkey

Application

WB,IHC

Application Image

Recommended Dilution

WB 1:2000
IHC 1:50

Mol Weight

47.2 kDa

Source

Mouse

Immunogen

Full-length protein expressed in 293T cell transfected with human IRF3 expression vector

Clonality

Monoclonal

Purification

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Buffer

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage

Store at -20 °C. Stable for 12 months from date of receipt.

	Western blot analysis of extracts(35ug) from 4 different cell lines and human plasma by using anti-IRF3 monoclonal antibody(1:500).
	Equivalent amounts of cell lysates(10 ug per lane) of wild-type HeLa cells and IRF3-Knockout HeLa cells(KO) were separated by SDS-PAGE and immunoblotted with anti-IRF3 monoclonal antibody MG623921(1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
	Western blot analysis of extracts(35ug) from 9 different cell lines by using anti-anti-IRF3monoclonal antibody.
	HEK293T cells were transfected with the pCMV6-ENTRY control(Left lane) or pCMV6-ENTRY IRF3(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates(5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3(Cat# MG623921).

	Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-IRF3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-IRF3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-IRF3mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG623921,Dilution 1:50)