PDE4A Antibody_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

MG624648

货号规格价格(￥)

MG624648S 50μl

MG624648M 100μl

以上产品均有现货供应

订购咨询： orders@ab-mart.com

订货热线： 4006-123-828

技术支持：15618194176

经销商名录：点击查看

Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

ABMART实验方案下载

经典一抗-PDE4A Antibody

Datasheet

Description

Hydrolyzes the second messenger 3'',5''-cyclic AMP (cAMP), which is a key regulator of many important physiological processes.; [Isoform 1]: Efficiently hydrolyzes cAMP.; [Isoform 2]: Efficiently hydrolyzes cAMP.; [Isoform 3]: Efficiently hydrolyzes cAMP. The phosphodiesterase activity is not affected by calcium, calmodulin or cyclic GMP (cGMP) levels. Does not hydrolyze cGMP.; [Isoform 4]: Efficiently hydrolyzes cAMP.; [Isoform 6]: Efficiently hydrolyzes cAMP.; [Isoform 7]: Efficiently hydrolyzes cAMP.

Alternative Names

3'',5''-cyclic-AMP phosphodiesterase 4A (EC 3.1.4.53) (DPDE2) (PDE46) (cAMP-specific phosphodiesterase 4A)
PDE4A DPDE2 DPDE2; PDE4; PDE46

UniProt

P27815

Reactivity

Human, Rat

Application

WB,IHC,IF,ICC,FC

Application Image

Recommended Dilution

WB 1:2000
IHC 1:50
IF 1:100
ICC 1:100-1:400
FC 1:100

Mol Weight

72.0 kDa

Source

Mouse

Immunogen

Full length human recombinant protein of human PDE4A (NP_006193) produced in HEK293T cell.

Clonality

Monoclonal

Purification

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Buffer

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage

Store at -20 °C. Stable for 12 months from date of receipt.

HEK293T cells were transfected with the pCMV6-ENTRY control(Left lane) or pCMV6-ENTRY PDE4A(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates(5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A(Cat# MG624648).

	Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody(MG624648).
	Anti-PDE4A mouse monoclonal antibody(MG624648) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4A().

	Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDE4A mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG624648,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PDE4A mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG624648,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE4A mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG624648,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PDE4A mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG624648,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PDE4A mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG624648,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE4A mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG624648,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDE4A mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG624648,Dilution 1:50)
	Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PDE4A mouse monoclonal antibody.(Heat-induced epitope retrieval by 10mM citric buffer,pH6.0,100℃ for 10min,MG624648,Dilution 1:50)

HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE4A antibody(MG624648),and then analyzed by flow cytometry.