WTAP Antibody_经典一抗 -

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目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
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细胞因子
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MG931773

货号规格价格(￥)

MG931773S 50μl

MG931773M 100μl

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-WTAP Antibody

Datasheet

Description

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3'' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).

Alternative Names

Pre-mRNA-splicing regulator WTAP (Female-lethal(2)D homolog) (hFL(2)D) (WT1-associated protein) (Wilms tumor 1-associating protein)
WTAP KIAA0105 Mum2

UniProt

Q15007

Reactivity

Human, Mouse, Rat

Application

WB,IHC

Application Image

Recommended Dilution

WB 1:2000
IHC 1:150

Mol Weight

44.1 kDa

Source

Mouse

Immunogen

Human recombinant protein fragment corresponding to amino acids 2-106 of human WTAP(NP_004897) produced in E.coli.

Clonality

Monoclonal

Purification

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Buffer

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage

Store at -20 °C. Stable for 12 months from date of receipt.

HEK293T cells were transfected with the pCMV6-ENTRY control(Left lane) or pCMV6-ENTRY WTAP(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates(5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WTAP(Cat# MG931773)(1:2000). Positive lysates can be purchased separately from Abmart.

	Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Human tonsil using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer(pH8.5) at 120℃ for 3min,MG931773)(1:150)