Involved in microhomology-mediated end-joining (MMEJ) DNA repair by promoting recruitment of polymerase theta (POLQ) to DNA damage sites during mitosis (PubMed:37440612). MMEJ is an alternative non-homologous end-joining (NHEJ) machinery that takes place during mitosis to repair double-strand breaks in DNA that originate in S-phase (PubMed:37440612). Accumulates in M-phase; following phosphorylation by PLK1, interacts with POLQ, enabling its recruitment to double-strand breaks for subsequent repair (PubMed:37440612). Also involved in the DNA damage response (DDR) signaling in response to genotoxic stresses such as ionizing radiation (IR) during the S phase (PubMed:21659603, PubMed:25602520). Recruited to sites of DNA damage through interaction with the 9-1-1 cell-cycle checkpoint response complex and TOPBP1 in a ATR-dependent manner (PubMed:21659603, PubMed:25602520). Required for the progression of the G1 to S phase transition (PubMed:21659603). Plays a role in the stimulation of CHEK1 phosphorylation (PubMed:21659603).

RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 (RAD9, RAD1, HUS1-interacting nuclear orphan protein)
RHNO1 C12orf32 RHINO HKMT1188 chromosome 12 open reading frame 32; HKMT1188; HKMT1188, MGC13204; MGC13204

Q9BSD3

Human

WB

WB 1:500

26.5 kDa

Mouse

Full length human recombiant protein of human C12ORF32 (NP_113653) produced in E.coli.

Monoclonal

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Store at -20 °C. Stable for 12 months from date of receipt.