MRPS34 Antibody_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

MG523546

货号规格价格(￥)

MG523546S 50μl

MG523546M 100μl

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-MRPS34 Antibody

Datasheet

Description

Required for mitochondrial translation, plays a role in maintaining the stability of the small ribosomal subunit and the 12S rRNA that are required for mitoribosome formation.

Alternative Names

Small ribosomal subunit protein mS34 (28S ribosomal protein S34, mitochondrial) (MRP-S34) (S34mt)
MRPS34 MRP-S12; MRP-S34; MRPS12

UniProt

P82930

Reactivity

Human, Mouse, Rat

Application

WB,IHC,IF,ICC,IP,ChIP

Application Image

Recommended Dilution

WB 1:1000-1:2000
IHC 1:100-200
IF 1:100-1:400
ICC 1:100-1:400
IP 1:50

Mol Weight

25.5 kDa

Source

Mouse

Immunogen

Full length human recombinant protein of human MRPS34(NP_076425) produced in E.coli.

Clonality

Monoclonal

Purification

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Buffer

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage

Store at -20 °C. Stable for 12 months from date of receipt.

	Western blot of cell lysates(35ug) from 9 different cell lines(1: HepG2,2: HeLa,3: SV-T2,4: A549. 5: COS7,6: Jurkat,7: MDCK,8: PC-12,9: MCF7).
	Western blot of human tissue lysates(15ug) from 10 different tissues(1: Testis,2: Omentum,3: Uterus,4: Breast,5: Brain,6: Liver,7: Ovary,8: Thyroid,9: Colon,10: Spleen). Diluation: 1:500.

Immunofluorescent staining of HeLa cells using anti-MRPS34 mouse monoclonal antibody(MG523546,green,1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin(red),and nuclear with DAPI(blue).

	Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MRPS34mouse monoclonal antibody.(MG523546; heat-induced epitope retrieval by 10mM citric buffer,pH6.0,120℃ for 3min)
	Immunohistochemical staining of paraffin-embedded human brain using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. MG523546 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in.
	Immunohistochemical staining of paraffin-embedded human breast normal adjacent using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. MG523546 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in breast epithelia cells.
	Immunohistochemical staining of paraffin-embedded human pituitary using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. MG523546 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in breast epithelia cells.
	Immunohistochemical staining of paraffin-embedded human liver normal adjacent using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. MG523546 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and puntate cytoplasmic staining in hepatocyte cells
	Immunohistochemical staining of paraffin-embedded human kidney normal adjacent using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. MG523546 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in the normal kidney epithelia cells.
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MRPS34 mouse monoclonal antibody.(MG523546; heat-induced epitope retrieval by 10mM citric buffer,pH6.0,120℃ for 3min)
	Immunohistochemical staining of paraffin-embedded human thyroid cancer using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. MG523546 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in tumor cells.
	Immunohistochemical staining of paraffin-embedded human lung cancer using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. MG523546 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in tumor cells.
	Immunohistochemical staining of paraffin-embedded human colon normal adjacent using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. MG523546 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in the normal epithelia cells.
	Immunohistochemical staining of paraffin-embedded human colon cancer using anti-MRPS34 clone UMAB125 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. MG523546 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in the tumor cells.
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPS34 mouse monoclonal antibody.(MG523546; heat-induced epitope retrieval by 10mM citric buffer,pH6.0,120℃ for 3min)