Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance . Citrullinates histone H1 at ''Arg-54'' (to form H1R54ci), histone H3 at ''Arg-2'', ''Arg-8'', ''Arg-17'' and/or ''Arg-26'' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at ''Arg-3'' (to form H4R3ci) . Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of ''Arg-54'' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance . Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci . Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription . Citrullinates EP300/P300 at ''Arg-2142'', which favors its interaction with NCOA2/GRIP1 .

PADI4;PAD; PAD4; PADI5; PDI4; PDI5

q9um07

Human

WB,IHC,IF,ICC

WB 1:2000
IHC 1:150
IF 1:100
ICC 1:100-1:400

Monoclonal

73.9 kDa

Mouse

Human recombinant protein fragment corresponding to amino acids 299-588 of human PADI4(NP_036519) produced in E.coli.

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.