Deubiquitinase essential for the regulation of nucleolar structure and function . Required for cell and organism viability . Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability . Functions as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 ''Lys-4'' trimethylation (H3K4) . Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF(FBW7) complex . In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm . Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions . Deubiquitinates SOD2, regulates SOD2 protein stability . Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins . Promotes CEP63 stabilization through ''Lys-48''-linked deubiquitination leading to increased stability . Acts as a SUMO ligase to promote EXOSC10 sumoylation critical for the nucleolar RNA exosome function in rRNA processing . Binds to pre-rRNAs .

USP36;DUB1

q9p275

Human

WB,IHC

WB 1:2000
IHC 1:150

Monoclonal

122.7 kDa

Mouse

Human recombinant protein fragment corresponding to amino acids 589-972 of human USP36 (NP_079366) produced in E.coli.

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.