E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications, cell cycle and DNA repair . Acts as a specific reader for 5-hydroxymethylcytosine (5hmC) and thereby recruits various substrates to these sites to ubiquitinate them . This activity also allows the maintenance of 5mC levels at specific genomic loci and regulates neuron-related gene expression (By similarity). Participates in cell cycle regulation by ubiquitinating cyclins CCND1 and CCNE1 and thereby inducing G1 arrest . Ubiquitinates also PCNP leading to its degradation by the proteasome . Plays an active role in DNA damage repair by ubiquitinating p21/CDKN1A leading to its proteasomal degradation . Promotes also DNA repair by acting as an interstrand cross-links (ICLs) sensor. Mechanistically, cooperates with UHRF1 to ensure recruitment of FANCD2 to ICLs, leading to FANCD2 monoubiquitination and subsequent activation . Contributes to UV-induced DNA damage response by physically interacting with ATR in response to irradiation, thereby promoting ATR activation .

NIRF;NIRF; RNF107; TDRD23; URF2

q96pu4

Human,Mouse,Rat

WB

WB 1:500-2000

Monoclonal

89.8 kDa

Mouse

Human recombinant protein fragment corresponding to amino acids 1-288 of human UHRF2(NP_690856) produced in E.coli.

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.