Serine/threonine kinase that regulates several signal transduction pathways . Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes . Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription . Phosphorylates ''Thr-18'' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1 . Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins . Down-regulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1 . Blocks the phosphorylation of ERK in response to ERBB2 and HRAS . Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant .; [Isoform 2]: Phosphorylates ''Thr-18'' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity.

VRK2;vaccinia-related kinase-2; vaccinia related kinase 2; vaccinia virus B1R-related kinase 2

q86y07

Human

WB

WB 1:500-2000

Monoclonal

Mouse

Human recombinant protein fragment corresponding to amino acids 301-486 of human VRK2 (NP_006287) produced in E.coli.

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.