Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues . Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5''-YCAAGGYC-3'' and 5''-RRAGGTCA-3'' are the consensus sequences for the recognition by NR5A1 . The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.

Steroidogenic Factor 1;AD4BP; ELP; FTZ1; FTZF1; hSF-1; POF7; SF-1; SF1; SPGF8; SRXY3

q13285

Human,Mouse,Rat

WB

WB 1:500

Monoclonal

51.5 kDa

Mouse

Human recombinant protein fragment corresponding to amino acids 1-262 of human NR5A1(NP_004950) produced in E.coli.

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.