Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA . Required for double-strand break repair and V(D)J recombination . Also has a role in chromosome translocation . Has a role in chromosome translocation . The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner . It works in the 3''-5'' direction . During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection . Binding to DNA may be mediated by XRCC6 . The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold . The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together . The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step . Probably also acts as a 5''-deoxyribose-5-phosphate lyase (5''-dRP lyase), by catalyzing the beta-elimination of the 5'' deoxyribose-5-phosphate at an abasic site near double-strand breaks . 5''-dRP lyase activity allows to ''clean'' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined . The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription . In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression . Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway . Negatively regulates apoptosis by interacting with BAX and sequestering it from the mitochondria . Might have deubiquitination activity, acting on BAX . .

X-ray repair cross-complementing protein 6 (EC 3.6.4.-) (EC 4.2.99.-) (5''-deoxyribose-5-phosphate lyase Ku70) (5''-dRP lyase Ku70) (70 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit) (CTC box-binding factor 75 kDa subunit) (CTC75) (CTCBF) (DNA repair protein XRCC6) (Lupus Ku autoantigen protein p70) (Ku70) (Thyroid-lupus autoantigen) (TLAA) (X-ray repair complementing defective repair in Chinese hamster cells 6),XRCC6 G22P1

P12956,P23475

2547

Human,Rat,Mouse

WB,IHC,ELISA

WB 1:500-2000
IHC 1:50-300
ELISA 2000-20000

The antibody detects endogenous Ku-70 when Acetyl occurs at Lys317

69kDa

Rabbit

Synthetic Acetyl peptide from human protein.

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.