Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest . Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts (By similarity). Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding . Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes (By similarity). .

Cyclin-dependent kinase inhibitor 1 (CDK-interacting protein 1) (Melanoma differentiation-associated protein 6) (MDA-6) (p21),CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1

P38936,P39689

1026/12575

Human, Mouse

WB,IHC,IF,ICC,ELISA

WB 1:500-2000
IHC 1:100-400
IF 1:100-500
ICC 1:100-500
ELISA 1:1000-5000

This antibody detects endogenous levels of p21 protein.

21kDa

Mouse

Synthesized peptide derived from human p21 AA range: 1-100

Monoclonal

Multiclone

IgG1,Kappa

Protein G

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.