Heterochromatin component that specifically recognizes and binds methylated ''Lys-9'' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression . Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 . Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene . Mediates down-regulation of CDH1 expression . Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression . The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed . .

M-phase phosphoprotein 8 (Two hybrid-associated protein 3 with RanBPM) (Twa3),MPHOSPH8 MPP8

Q99549,Q3TYA6,G3V8T1

54737

Human,Rat,Mouse

WB,ELISA

WB 1:500-2000
ELISA 1:5000-20000

MPP8 Polyclonal Antibody detects endogenous levels of protein.

94kDa

Rabbit

Synthesized peptide derived from human protein.

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.