DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) . Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3'' guanine . Particularly important for the repair of UV-induced pyrimidine dimers . Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes . Forms a Schiff base with 5''-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5''-deoxyribose phosphate (5''-dRP) residue. This covalent trapping of the enzyme by the 5''-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis . Targets POLI to replication foci . .

DNA polymerase eta (EC 2.7.7.7) (RAD30 homolog A) (Xeroderma pigmentosum variant type protein),POLH RAD30 RAD30A XPV

Q9Y253,Q9JJN0

5429

Human,Mouse

WB,ELISA

WB 1:500-2000
ELISA 1:5000-20000

POLH Polyclonal Antibody detects endogenous levels of protein.

78kDa

Rabbit

Synthesized peptide derived from part region of human protein

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.