Multifunctional enzyme that has magnesium and ATP-dependent 3''-5'' DNA-helicase activity on partially duplex substrates . Also has 3''->5'' exonuclease activity towards double-stranded (ds)DNA with a 5''-overhang . Has no nuclease activity towards single-stranded (ss)DNA or blunt-ended dsDNA . Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency; strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3) . Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation . Unwinds some G-quadruplex DNA (d(CGG)n tracts); unwinding seems to occur in both 5''-3'' and 3''-5'' direction and requires a short single-stranded tail . d(CGG)n tracts have a propensity to assemble into tetraplex structures; other G-rich substrates from a telomeric or IgG switch sequence are not unwound . Depletion leads to chromosomal breaks and genome instability . .

Bifunctional 3''-5'' exonuclease/ATP-dependent helicase WRN (DNA helicase, RecQ-like type 3) (RecQ protein-like 2) (Werner syndrome protein) [Includes: 3''-5'' exonuclease (EC 3.1.-.-); ATP-dependent helicase (EC 5.6.2.4) (DNA 3''-5'' helicase WRN)],WRN RECQ3 RECQL2

Q14191,O09053

7486

Human,Rat,Mouse

WB,ELISA

WB 1:500-2000
ELISA 1:5000

Phospho-WRN (S1141) Polyclonal Antibody detects endogenous levels of WRN protein only when phosphorylated at S1141.

162kDa

Rabbit

Synthesized peptide derived from human Werner Syndrome Helicase around the phosphorylation site of Ser1141.

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.