Nuclease involved in DNA non-homologous end joining (NHEJ); required for double-strand break repair and V(D)J recombination . Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments . V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs) . These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends . These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively . This protein exhibits single-strand specific 5''-3'' exonuclease activity in isolation and acquires endonucleolytic activity on 5'' and 3'' hairpins and overhangs when in a complex with PRKDC . The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint . Also required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ . .

Protein artemis (EC 3.1.-.-) (DNA cross-link repair 1C protein) (Protein A-SCID) (SNM1 homolog C) (hSNM1C) (SNM1-like protein),DCLRE1C ARTEMIS ASCID SCIDA SNM1C

Q96SD1,Q8K4J0

64421

Human,Rat,Mouse

IHC,IF,ICC,ELISA

IHC 1:50-300
IF 1:50-200
ICC 1:50-200
ELISA 1:10000

Artemis Polyclonal Antibody detects endogenous levels of Artemis protein.

Rabbit

The antiserum was produced against synthesized peptide derived from human Artemis. AA range:482-531

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.