Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (By similarity). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins . Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels . Stimulates the activity of receptor-regulated K(+) channels . The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division . The active GTP-bound form activates the calcium permeant TRPC5 ion channels . .

Guanine nucleotide-binding protein G(i) subunit alpha-3 (G(i) alpha-3),GNAI3

P08754,Q9DC51,P08753

2773/14679/25643

Human,Mouse,Rat

WB,ELISA

WB 1:500-2000
ELISA 1:40000

Gα i-3 Polyclonal Antibody detects endogenous levels of Gα i-3 protein.

41kDa

Rabbit

The antiserum was produced against synthesized peptide derived from human GNAI3. AA range:31-80

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.