Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA . Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated . Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors . Recognizes and binds histone H3 crotonylated at ''Lys-9'' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at ''Lys-18'' (H3K18cr) . Also recognizes and binds histone H3 acetylated and butyrylated at ''Lys-9'' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3 . In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones . Recruitment of the SEC complex to crotonylated histones promotes recruitment of DOT1L on active chromatin to deposit histone H3 ''Lys-79'' methylation (H3K79me) . Plays a key role in hematopoietic stem cell (HSC) maintenance by preserving, rather than conferring, HSC stemness . Acts by binding to the transcription start site of active genes in HSCs and sustaining level of H3K79me2, probably by recruiting DOT1L . .

Protein AF-9 (ALL1-fused gene from chromosome 9 protein) (Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein) (YEATS domain-containing protein 3),MLLT3 AF9 YEATS3

P42568,A2AM29

4300/70122

Human,Mouse

WB

WB 1:500-2000

This antibody detects endogenous levels of AF9 at Human/Mouse

60kDa

Rabbit

Synthesized peptide derived from human AF9 AA range: 328-378

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.