Histone methyltransferase that plays an essential role in early development and hematopoiesis . Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of ''Lys-4'' of histone H3 (H3K4me) complex and acetylation of ''Lys-16'' of histone H4 (H4K16ac) . Catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of ''Lys-4'' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place . Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity . Has no activity toward histone H3 phosphorylated on ''Thr-3'', less activity toward H3 dimethylated on ''Arg-8'' or ''Lys-9'', while it has higher activity toward H3 acetylated on ''Lys-9'' . Binds to unmethylated CpG elements in the promoter of target genes and helps maintain them in the nonmethylated state . Required for transcriptional activation of HOXA9 . Promotes PPP1R15A-induced apoptosis . Plays a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-BMAL1 heterodimer (By similarity). Establishes a permissive chromatin state for circadian transcription by mediating a rhythmic methylation of ''Lys-4'' of histone H3 (H3K4me) and this histone modification directs the circadian acetylation at H3K9 and H3K14 allowing the recruitment of CLOCK-BMAL1 to chromatin (By similarity). Also has auto-methylation activity on Cys-3882 in absence of histone H3 substrate . .

Histone-lysine N-methyltransferase 2A (Lysine N-methyltransferase 2A) (EC 2.1.1.364) (ALL-1) (CXXC-type zinc finger protein 7) (Cysteine methyltransferase KMT2A) (EC 2.1.1.-) (Myeloid/lymphoid or mixed-lineage leukemia) (Myeloid/lymphoid or mixed-lineage leukemia protein 1) (Trithorax-like protein) (Zinc finger protein HRX) [Cleaved into: MLL cleavage product N320 (N-terminal cleavage product of 320 kDa) (p320); MLL cleavage product C180 (C-terminal cleavage product of 180 kDa) (p180)],KMT2A ALL1 CXXC7 HRX HTRX MLL MLL1 TRX1

Q03164,P55200

4297/214162

Human,Mouse,Rat

WB,IHC,IF,ICC,ELISA

WB 1:500-2000
IHC 1:50-300
IF 1:50-200
ICC 1:50-200
ELISA 1:10000

The antibody detects endogenous MLL

Rabbit

Synthetic peptide from human protein.

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.