Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis . Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 . In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites . Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at ''Lys-15'' (H2AK15Ub) and histone H4 dimethylated at ''Lys-20'' (H4K20me2), two histone marks that are present at DSBs sites . Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs . Participates in the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 . .

TP53-binding protein 1 (53BP1) (p53-binding protein 1) (p53BP1),TP53BP1

Q12888,P70399

7158/27223

Human,Rat,Mouse

WB

WB 1:500-2000

This antibody detects endogenous levels of Human 53BP1 (phospho-Thr543)

213kDa

Rabbit

Synthesized phosho peptide around human 53BP1 (Thr543)

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.