Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription . Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition (By similarity). Degradation of CDKN1B/p27kip also requires CKS1 (By similarity). Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, NBN, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1 . Degradation of TAL1 also requires STUB1 . Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2 . Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration . Following phosphorylation in response to DNA damage, mediates ''Lys-63''-linked ubiquitination of NBN, promoting ATM recruitment to DNA damage sites and DNA repair via homologous recombination . .; Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus. .

S-phase kinase-associated protein 2 (Cyclin-A/CDK2-associated protein p45) (F-box protein Skp2) (F-box/LRR-repeat protein 1) (p45skp2),SKP2 FBXL1

Q13309,Q9Z0Z3

6502/27401

Human,Rat,Mouse

WB,IHC,ELISA

WB 1:500-2000
IHC 1:50-300
ELISA 2000-20000

This antibody detects endogenous levels of Human Skp2 (phospho-Ser64)

47kDa

Rabbit

Synthesized phosho peptide around human Skp2 (Ser64)

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.