Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation . Involved in an early step of the formation of preautophagosomal structures . Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases . Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P . Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane . .; [Isoform 4]: Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella. .

WD repeat domain phosphoinositide-interacting protein 2 (WIPI-2) (WIPI49-like protein 2),WIPI2 CGI-50

Q9Y4P8,Q80W47,Q6AY57

26100/74781/288498

Human,Mouse,Rat

WB

WB 1:500-2000

This antibody detects endogenous levels of Human Mouse Rat WIPI2 (phospho-Ser413)

49kDa

Rabbit

Synthesized phosho peptide around human WIPI2 (Ser413)

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.