E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, leading to degradation of nascent peptide chains . ZNF598 is activated when ribosomes are stalled within an mRNA following translation of prematurely polyadenylated mRNAs . Acts as a ribosome collision sensor: specifically recognizes and binds collided di-ribosome, which arises when a trailing ribosome encounters a slower leading ribosome, leading to terminally arrest translation . Following binding to colliding ribosomes, mediates monoubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS3/uS3, and ''Lys-63''-linked polyubiquitination of RPS20/uS10 . Polyubiquitination of RPS20/uS10 promotes recruitment of the RQT (ribosome quality control trigger) complex, which drives the disassembly of stalled ribosomes, followed by degradation of nascent peptides . E3 ubiquitin-protein ligase activity is dependent on the E2 ubiquitin-conjugating enzyme UBE2D3 . Also acts as an adapter that recruits the 4EHP-GYF2 complex to mRNAs . Independently of its role in RQC, may also act as a negative regulator of interferon-stimulated gene (ISG) expression . .; (Microbial infection) Required for poxvirus protein synthesis by mediating ubiquitination of RPS10/eS10 and RPS20/uS10 . Poxvirus encoding mRNAs contain unusual 5'' poly(A) leaders and ZNF598 is required for their translational efficiency, possibly via its ability to suppress readthrough or sliding on shorter poly(A) tracts . .

E3 ubiquitin-protein ligase ZNF598 (EC 2.3.2.27) (Zinc finger protein 598),ZNF598

Q86UK7,Q80YR4

90850/213753

Human,Mouse

WB,ELISA

WB 1:500-2000
ELISA 1:5000-20000

This antibody detects endogenous levels of Human,Mouse ZN598

99kDa

Rabbit

Synthesized peptide derived from human ZN598

Polyclonal

Rabbit,IgG

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.