Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes . The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine . Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress . LRRC8A and LRRC8D are required for the uptake of the drug cisplatin . In complex with LRRC8C or LRRC8E, acts as a transporter of immunoreactive cyclic dinucleotide GMP-AMP (2''-3''-cGAMP), an immune messenger produced in response to DNA virus in the cytosol: mediates both import and export of 2''-3''-cGAMP, thereby promoting transfer of 2''-3''-cGAMP to bystander cells . In contrast, complexes containing LRRC8D inhibit transport of 2''-3''-cGAMP . Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition . Can form functional channels by itself (in vitro) . Involved in B-cell development: required for the pro-B cell to pre-B cell transition . Also required for T-cell development (By similarity). Required for myoblast differentiation: VRAC activity promotes membrane hyperpolarization and regulates insulin-stimulated glucose metabolism and oxygen consumption (By similarity). Also acts as a regulator of glucose-sensing in pancreatic beta cells: VRAC currents, generated in response to hypotonicity- or glucose-induced beta cell swelling, depolarize cells, thereby causing electrical excitation, leading to increase glucose sensitivity and insulin secretion . Also plays a role in lysosome homeostasis by forming functional lysosomal VRAC channels in response to low cytoplasmic ionic strength condition: lysosomal VRAC channels are necessary for the formation of large lysosome-derived vacuoles, which store and then expel excess water to maintain cytosolic water homeostasis . Acts as a key factor in NLRP3 inflammasome activation by modulating itaconate efflux and mitochondria function . .

Volume-regulated anion channel subunit LRRC8A (Leucine-rich repeat-containing protein 8A) (HsLRRC8A) (Swelling protein 1),LRRC8A KIAA1437 LRRC8 SWELL1 UNQ221/PRO247

Q8IWT6,Q80WG5,Q4V8I7

56262/241296/311846

Human,Mouse,Rat

WB

WB 1:500-2000

This antibody detects endogenous levels of LRRC8A/SWELL1 at Human, Mouse,Rat

89kDa

Rabbit

Polyclonal

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

1 mg/ml

Store at -20℃. Stable for 12 months from date of receipt.